You Searched For: UV\/Visible Spectrophotometers

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Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

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Click to edit Master title style Got Protein? Testing the protein content of common foods Bradford Protein Assay Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

LABORATORY CHECKLIST Microscopy ☐ Bottles Media* Sampling* Wash ☐ Bulbs ☐ Cell Counters ☐ Cleaners/Disinfectants ☐ Dissecting Tools ☐ Eyewear ☐ Fluorometers ☐ Gloves Chemical General Use ☐ Hemacytometers Disposable Reusable ☐ Immersion Oil ☐ Lab C...

Using Type 1 Water (18.2 MΩ-cm) for High Performance Liquid Chromatography in the Food & Beverage Industry Tammy Goodman Commercial Manager Water Purification Thermo Scientific High Performance Liquid Chromatography (HPLC) is widely used in the fo...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

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