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Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Click to edit Master title style Got Protein? Testing the protein content of common foods Bradford Protein Assay Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller...

LABORATORY CHECKLIST Microscopy ☐ Bottles Media* Sampling* Wash ☐ Bulbs ☐ Cell Counters ☐ Cleaners/Disinfectants ☐ Dissecting Tools ☐ Eyewear ☐ Fluorometers ☐ Gloves Chemical General Use ☐ Hemacytometers Disposable Reusable ☐ Immersion Oil ☐ Lab C...

Microscopy ☐ Bottles Media* Sampling* Wash ☐ Bulbs ☐ Cell Counters ☐ Cleaners/Disinfectants ☐ Dissecting Tools ☐ Eyewear ☐ Fluorometers ☐ Gloves Chemical General Use ☐ Hemacytometers Disposable Reusable ☐ Immersion Oil ☐ Lab Coats ☐ Labels and Tap...

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Azure Biosystems is dedicated to designing instruments that can deliver industry-leading performance across a broad range of laboratory applications without overcomplicating the user experience. We have designed a suite of upgradeable cSeries inst...

PowerPoint Presentation pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP) Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller Education Cente...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagents and master mixes pipetting error is accommodated for. What is not typically discussed or published are the causes and ramific...

TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagent cocktails pipetting error is accommodated for. What is not typically discussed or published are the causes and ramifications o...

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