This simulation introduces students to the use of DNA fingerprinting.
- Real-World Connection
- Time Required: 45 Minutes
- Simulation Involves Paternity Determination
- Basic Concepts of DNA Testing Instruction
- Instructions Included
This hypothetical paternity determination activity compares the DNA of a child with DNA from two possible fathers to find a biological match.
The experiment is an excellent way to teach fundamentals and practical applications of DNA electrophoresis as well as one of the most compelling and difficult social issues to arise from DNA testing.
Ordering information: Required but not included: Transfer pipets (automatic micropipettes with tips and pipet pump), balance; microwave, hotplate or burner; 250 ml flasks or beakers, hot gloves, safety goggles and disposable laboratory gloves, small plastic trays or large weigh boats (for gel destaining), DNA visualization system (white light), distilled or deionized water, horizontal gel electrophoresis apparatus, and D.C. power supply.
Delivery information: This simulation includes instructions, ready-to-load DNA, agarose, practice gel-loading solution, electrophoresis buffer, InstaStain Blue, and FlashBlue stain.
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